We provide Self-contained biological indicator to monitor EO/EtO  (ethylene oxide) sterilization. It contains bacterial spores on a paper carrier packaged within a small, thermoplastic culture tube. Inside the thermoplastic culture tube is a sealed glass ampoule of specially formulated soybean casein digest culture medium containing a color indicator which turns a dramatic yellow when spores grow. The our self-contained biological indicator is easy-to-use and produces visual results within 48 hours without laboratory transfers.

Simply place the biological indicator inside products or product packages to be sterilized. The “inoculated package” is then placed in the sterilizer and processed. Upon removal from the sterilizer, the biological indicator is “activated”. Using a crushing device, flex the sides of the soft plastic culture tube, break the glass ampoule allowing the medium to mix with the spores. The “activated” biological indicator is then placed in an appropriate incubator for 24 hours (steam) or 48 hours (EtO). If any spores survive the process, the medium will turn yellow.